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Domain > tissuegrown.com
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Date
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2025-12-08
50.63.8.195
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Port 80
HTTP/1.1 301 Moved PermanentlyContent-Type: text/html; charsetUTF-8Location: https://tissuegrown.com/Server: Microsoft-IIS/10.0X-Powered-By: ASP.NETX-Powered-By-Plesk: PleskWinDate: Mon, 08 Dec 2025 05:36:44 GMTContent-Length: 147 head>title>Document Moved/title>/head>body>h1>Object Moved/h1>This document may be found a HREFhttps://tissuegrown.com/>here/a>/body>
Port 443
HTTP/1.1 200 OKCache-Control: privateContent-Type: text/html; charsetutf-8Server: Microsoft-IIS/10.0X-AspNetMvc-Version: 3.0X-AspNet-Version: 4.0.30319X-Powered-By: ASP.NETX-Powered-By-Plesk: PleskWinDate: Mon, 08 Dec 2025 05:36:44 GMTContent-Length: 125508 html xmlnshttp://www.w3.org/1999/xhtml dirltr langen> head> meta http-equivcontent-type contenttext/html; charsetUTF-8 /> meta nameapple-mobile-web-app-capable contentyes /> link relSHORTCUT ICON href/Content/favicon.ico> meta http-equivX-UA-Compatible contentIEedge> meta nameviewport contentwidthdevice-width, heightdevice-height, initial-scale1> meta namereferrer contentorigin> meta propertyog:type contentwebsite> meta propertyog:url contenthttps://tissuegrown.com/> meta propertyog:image itempropimage primaryImageOfPage contenthttp://www.tissuegrown.com/Content/Walnut Tree bg.png> meta nametwitter:card contentsummary/> meta nametwitter:domain contenttissuegrown.com/> meta nametwitter:title propertyog:title itemproptitle name contentWelcome to Tissue-Grown Corporation /> meta nametwitter:description propertyog:description itempropdescription contentTissue-Grown Corporation, Santa Paula, California Is A Plant Tissue Culture Company Specialized In Disease Elite, High Volume, Plant Propagation And Tissue Culture Services. /> meta namedescription contentTissue-Grown Corporation, Santa Paula, California Is A Plant Tissue Culture Company Specialized In Disease Elite, High Volume, Plant Propagation And Tissue Culture Services.> meta namekeywords contentplant tissue culture, lab, pistachio rootstock, walnut rootstock, plantlet, micropropagation, in vitro, virus elite, disease elite, plant propagation, california, disease-indexed, horticulture, germplasm> title>Welcome to Tissue-Grown Corporation/title> script typeapplication/ld+json> { @context: http://schema.org/, @type: Corporation, address: { @type: PostalAddress, addressCountry: United States, postalCode: 93060, streetAddress: 15245 W. Telegraph Rd., telephone: 8055251975 }, legalName: Tissue-Grown Corporation, description: Tissue-Grown Corporation, Santa Paula, California Is A Plant Tissue Culture Company Specialized In Disease Elite, High Volume, Plant Propagation And Tissue Culture Services., mainEntityOfPage: https://tissuegrown.com/, name: Welcome to Tissue-Grown Corporation } /script> script src/Scripts/jquery-1.11.min.js>/script> script src/Scripts/jquery-ui.min.js>/script> script> $.fn.transform function (value) { return this.css(transform, value); }; var stopLoading false; var loadItem localStorage.getItem(loaded); var loaded Date.now() - loadItem 1000 * 60 * 60 * 24 * 7 ? 1 : 0;// one week $(function () { if (loaded) { $(.splashscreen).remove(); return; } $(.splashscreen).css(top, 0); $.fn.reveal function (offset) { var s $(span, this).eq(offset); s.css(opacity, 1); setTimeout(function () { s.css(color, white); }, 100) return this; }; $(.logocover).css(transform, translateX(100%)); var name Tissue-Grown Corporation; var lastCall; var letterIndex 0; (function draw(timestamp) { if (stopLoading) return; if (!lastCall) lastCall timestamp; if (timestamp - lastCall > 60) { $(.intro).reveal(letterIndex++); if (letterIndex name.length) { return; } lastCall timestamp; } window.requestAnimationFrame(draw); })(); (function anim(sq, curr) { if (!$(.splashscreen).is(:visible) || stopLoading) return; curr.animate({ opacity: 1 }, medium, function () { var prev curr; curr curr.next(); if (!curr.length) curr sq.eq(0); prev.animate({ opacity: 0 }); anim(sq, curr); }); })($(.squares div), $(.squares div).eq(0)); }); $(window).on(load, function () { $(function () { $(.splashscreen).fadeOut(function () { $(this).remove(); }); stopLoading true; localStorage.setItem(loaded, Date.now()); var lastStart 0; var firstStart 0; var firstTime Date.now(); var lastTime Date.now(); var hook document.body; var hookHeight $(hook).height(); var hookWidth $(hook).width(); var locked false; var movesAvail 1; var directionLock false; var currentDirection; var velocity 0; var lastScroll 0; var scrolling ; var autoscroll false; var xpos 0; //the page should stick to the finger, //and if there was a flick then it should flick in that direction //these are the only two expectactions of touch scrolling. $(hook).on(touchstart, function (e) { velocity 0; //switching to screenY due to a discrepency //lastStart firstStart lastScroll e.originalEvent.targetTouches0.pageY; lastStart firstStart lastScroll e.originalEvent.targetTouches0.screenY; lastTime firstTime e.timeStamp; xpos e.originalEvent.targetTouches0.screenX; }); $(hook).on(touchmove, function (e) { var thisTime e.timeStamp; var thisY e.originalEvent.targetTouches0.screenY; //switching to screenY due to a discrepency //var thisY e.originalEvent.targetTouches0.pageY; //distance is measured in screens var distance Math.abs(thisY - lastStart) / hookHeight; //height is 500 //thisY is 200 //lastStart is 400 //distance should be measured in the change over the screen //so abs(y-start) / height //would be a measurement of 0.4 (2/5 ths) screens //velocity is measured in screens per millisecond var time thisTime - lastTime; velocity + distance / time; //acceleration is measured in screens per millisecond per millisecond //current velocity - last velocity is the acceleration var pixels Math.abs(thisY - lastScroll); var scrolls parseInt(pixels / cardManager.pixelsPerScroll); if (scrolls > 0) { var dir thisY lastScroll ? down : up; //for (var i 0; i scrolls; i++) { // scrolling.push(setTimeout(function () { MouseWheelHandler(e, dir); // }, 50 * (i+1))); //} lastScroll thisY; } lastTime thisTime; //switching to screenY due to an android discrepency lastStart e.originalEvent.targetTouches0.screenY; //lastStart e.originalEvent.targetTouches0.pageY; }); $(hook).on(touchend, function (e) { var thisX e.originalEvent.changedTouches0.screenX; if (Math.abs(thisX - xpos) > (hookWidth * 0.27)) { //swipe if (thisX xpos) { //right $(.chevrighttarget:visible).click(); } else { //left $(.chevlefttarget:visible).click(); } return; } var thisTime e.timeStamp; var thisY e.originalEvent.changedTouches0.screenY; //distance is measured in screens var distance Math.abs(thisY - firstStart) / hookHeight; //height is 500 //thisY is 200 //lastStart is 400 //distance should be measured in the change over the screen //so abs(y-start) / height //would be a measurement of 0.4 (2/5 ths) screens //velocity is measured in screens per millisecond var allTime thisTime - firstTime; var averageAccel 1000000 * velocity / allTime;//screens per millisecond per millisecond (1M for /s/s) //150 is very fast, 15 is slow //we will say two 150s is one slide //so 150 4 scrolls... so every 35 is one scroll //that turned out to be a little slow, so we went with one 150 is one slide, so every 17 var dir thisY firstStart ? down : up; var accelScroll parseInt(averageAccel / 17); for (var i 0; i accelScroll; i++) { scrolling.push(setTimeout(function () { MouseWheelHandler(e, dir); }, Math.max(10, 50 * i))); } }); if (hook.addEventListener) { hook.addEventListener(mousewheel, MouseWheelHandler, false); hook.addEventListener(DOMMouseScroll, MouseWheelHandler, false); } else { hook.attachEvent(onmousewheel, MouseWheelHandler); } $(hook).keydown(function (e) { switch (e.which) { case 37: // left break; case 38: // up cardManager.scrollUp(); break; case 39: // right break; case 40: // down cardManager.scrollDown(); break; default: return; } }); var cms ; var cardManager; var shouldUpdate true; $(.icon).click(function () { if ($(this).hasClass(active)) return; var currentIndex cardManager.index; var nextName $(this).data(name); var nextManager cms.find(function (f) { return f.id nextName }); //var scrollSize 104 * (currentIndex - nextManager.index); //var scrollSize 100 * (currentIndex - nextManager.index); //if its negative, scroll left, positive scroll right^ //scroll left, means content moves left var slideDirection currentIndex nextManager.index ? -1 : 1; var slideDistance currentIndex - nextManager.index; //first slide if (nextManager.index 0) { $(divclass*chev-left).fadeOut(); $(.chevlefttarget).hide(); $(divclass*chev-right).fadeIn(); $(.chevrighttarget).show(); } //middle slide if (nextManager.index 1) { $(divclass*chev-left).fadeIn(); $(.chevlefttarget).show(); $(divclass*chev-right).fadeIn(); $(.chevrighttarget).show(); } //last slide if (nextManager.index 2) { $(divclass*chev-left).fadeIn(); $(.chevlefttarget).show(); $(divclass*chev-right).fadeOut(); $(.chevrighttarget).hide(); } for (var i 0; i cms.length; i++) { cmsi.slot + slideDistance;//slideDirection; var translate cmsi.slot 0 ? 0% : (cmsi.slot > 0 ? 107% : -107%); cmsi.cardHolder.transform(translateX( + translate + )); } cardManager nextManager; $(.active).removeClass(active); $(this).addClass(active); if (shouldUpdate) { cardManager.updatePageUrl(); } shouldUpdate true; }); $(.navlink).click(function () { var navTarget $(this).data(nav); var iconSelector .icon. + navTarget.split( )0; shouldUpdate false; $(iconSelector).click(); cardManager.scrollTo(navTarget.split( )1); }); $(.chevlefttarget).click(function () { $(.icon).eq(cardManager.index - 1).click(); }); $(.chevrighttarget).click(function () { $(.icon).eq(cardManager.index + 1).click(); }); $(divclass*chev-bottom).click(function () { cardManager.scrollDown(); }); $(.cardholder).each(function (i) { var cm new cardScroller($(this), i); cms.push(cm); if (i 0) cardManager cm; for (var n 0; n cms.length; n++) { var translate cmsn.slot 0 ? 0% : (cmsn.slot > 0 ? 107% : -107%); cmsn.cardHolder.transform(translateX( + translate + )); } }); var sizer; $(window).on(resize, function () { for (var i 0; i cms.length; i++) { cmsi.recalc(); } hookHeight $(hook).height(); hookWidth $(hook).width(); if (sizer) { clearTimeout(sizer); } sizer setTimeout(function () { for (var i 0; i cms.length; i++) { cmsi.recalc(); } hookHeight $(hook).height(); hookWidth $(hook).width(); }, 1000); }); $(window).on(orientationchange, function () { for (var i 0; i cms.length; i++) { cmsi.recalc(); } hookHeight $(hook).height(); hookWidth $(hook).width(); if (sizer) { clearTimeout(sizer); } sizer setTimeout(function () { for (var i 0; i cms.length; i++) { cmsi.recalc(); } hookHeight $(hook).height(); hookWidth $(hook).width(); }, 1000); }); //0 to -50, 4 steps, content out, every 12.5% //-50 to 0, 4 steps, content in function cardScroller(holder, index) { this.id holder0.id; this.index index; this.slot index; var cards this.cards $(.card, holder); var cardContents this.cardContents $(.cardtext, holder); var cardHolder this.cardHolder holder; var predictor $(.predictor); var textPredictor $(.textpredictor); var scrollIcon $(.scrollIcon); var scrollIndex this.scrollIndex 0; var mainCard this.cards.first(); var prevCard mainCard.prev(); var nextCard mainCard.next(); //the predictor needs to determine when the **end** on the bottom //of the zone is going to be hit cards.each(function (i) { var card this; this.data { top: i * 100, order: i, cardBottom: card.getBoundingClientRect().height, url: $(.cardTitle a, card).attr(href), title: $(.cardTitle a, card).text() }; }); cardContents.each(function () { var cardText this; this.data { top: 0, textHeight: cardText.getBoundingClientRect().height }; }); this.recalc function () { cards.each(function () { this.data.cardBottom this.getBoundingClientRect().height; }); cardContents.each(function () { this.data.textHeight this.getBoundingClientRect().height; }); this.calcPixelsPerScroll(); }; this.pixelsPerScroll 0; this.calcPixelsPerScroll function () { this.pixelsPerScroll cardHolder0.getBoundingClientRect().height * 0.0625; }; this.calcPixelsPerScroll(); var updatePageUrl this.updatePageUrl function () { window.history.replaceState(Tissue-Grown Corp, Tissue-Grown Corp - + mainCard0.data.title, mainCard0.data.url) }; this.scrollTo function (target, cb) { if (scrolling.length) { for (var i 0; i scrolling.length; i++) { clearTimeout(scrollingi); } scrolling ; } autoscroll true; var cm this; cb cb || function () { }; switch (target) { case first: (function first() { if (cm.cards0.data.top ! 0) { cm.scrollUp(); scrolling.push(setTimeout(first, 50)); } else { autoscroll false; cb(); } })() break; case last: (function last() { if (nextCard.length) { cm.scrollDown(); scrolling.push(setTimeout(last, 50)); } else { autoscroll false; cb(); } })() break; default: var tar cards.filter(target); if (tar.length && tar0 ! mainCard0) { var cardTxt $(.cardtext, tar); var dir tar0.data.order mainCard0.data.order ? scrollUp : scrollDown; var scrollDistance tar0.data.cardBottom / -8.25; var lastCall; (function to(timestamp) { if (!lastCall) lastCall timestamp; if (timestamp - lastCall > 50) { var txtTop cardTxt0.data.top; if (txtTop > scrollDistance && tar0.data.top 0) { autoscroll false; cb(); updatePageUrl(); return; } cmdir(); lastCall timestamp; } window.requestAnimationFrame(to); })(); } else { cb(); } break; } }; var firstDown true; var stepPercent 6.25; this.scrollDown function () { if (firstDown) { $(divclass*chev-bottom).remove(); firstDown false; } //scroll down, content up //move main card text in direction var cardText $(.cardtext, mainCard); var textHeight cardText0.data.textHeight; //var cardBottom mainCard.height();//rounding error var cardBottom mainCard0.data.cardBottom; var textTop cardText0.data.top; var textBottom Math.round(textTop + textHeight);//clientBoundingRect includes padding if (cardBottom textBottom) { //if the card is above the text var scrollDistance cardBottom / 8.25;//replacing the 15 var nextStep Math.min(scrollDistance, textTop + textHeight - cardBottom); //if nextStep is less than 15, snap it into place. if (nextStep scrollDistance) { cardText.transform(translateY( + (0 - (textHeight - cardBottom)) + px)); cardText0.data.top (0 - (textHeight - cardBottom)); } else { cardText.transform(translateY( + (textTop - nextStep) + px)); cardText0.data.top (textTop - nextStep); return; } } if (!nextCard.length && mainCard0.data.top 0) { //hit bottom, end scrolling for (var i 0; i scrolling.length; i++) { clearTimeout(scrollingi); } scrolling ; return; } //move main card up on the page var mainTop mainCard0.data.top; var change -stepPercent; mainCard.transform(translateY( + (mainTop + change) + %)); mainCard0.data.top mainTop + change; var cardbg $(.cardbg, mainCard); //cardbg.css(background-position, 0% + (-1 * mainCard0.data.top) + %); //cardbg.css(zoom, 1 - (mainCard0.data.top / (6.25 * 50))); //cardbg.finish().animate({ zoom: 1 - (mainCard0.data.top / (6.25 * 50)) }, 40); //cardbg.stop().animate({ zoom: 1 - (mainCard0.data.top / (6.25 * 50)) }, 40); //cardbg.css(transform,scale( + (1 - (mainCard0.data.top / (6.25 * 50))) + )); //move all next cards faster up on the page mainCard.nextAll(.card).each(function () { var next $(this); var nextTop next0.data.top; var nextChange -(stepPercent * 2); next.transform(translateY( + (nextTop + nextChange) + %)); next0.data.top nextTop + nextChange; }); //move all next card in towards main if (nextCard0 && nextCard0.data.top 0) { //change main //scrolling down meant next is new main prevCard mainCard; mainCard nextCard; if (!autoscroll) { this.updatePageUrl(); } nextCard mainCard.next(); this.cardsChanged true; } }; this.scrollUp function () { //scroll up, content down //check to see if content is overlapping from next to main if (nextCard.length && mainCard0.data.top ! 0 ) { //this was in use but was removed //card is in place } else { //scroll text first, dont move card if text is out of place var cardText $(.cardtext, mainCard); var scrollDistance mainCard0.data.cardBottom / 8.25; //is the slide partially scrolled? if (cardText0.data.top) { //if the text has a top setting of > 0 var textTop cardText0.data.top; var nextStep Math.max(0 - scrollDistance, textTop); cardText.transform(translateY( + (textTop - nextStep) + px)); cardText0.data.top textTop - nextStep; if (textTop ! nextStep) { return; } } } //so long as this isnt the top card //swap cards if the current one is already at the top since content needs to move down if (prevCard.length && mainCard0.data.top 0) {//change main //scrolling up meant prev is new main nextCard mainCard; mainCard prevCard; if (!autoscroll) { this.updatePageUrl(); } prevCard mainCard.prev(); this.cardsChanged true; } //dont scroll up if this is the first card if (!prevCard.length && mainCard0.data.top 0) { for (var i 0; i scrolling.length; i++) { clearTimeout(scrollingi); } scrolling ; return; } //move all next cards faster down on the page mainCard.nextAll(.card).each(function () { var next $(this); var nextTop next0.data.top; var nextChange stepPercent * 2; next.transform(translateY( + (nextTop + nextChange) + %)); next0.data.top nextTop + nextChange; }); //move main card down on the page var mainTop mainCard0.data.top; var change stepPercent; mainCard.transform(translateY( + (mainTop + change) + %));//110in,120out mainCard0.data.top mainTop + change; var cardbg $(.cardbg, mainCard); //cardbg.css(background-position, 0% + (-1 * mainCard0.data.top) + %); //cardbg.css(zoom, 1 - (mainCard0.data.top / (6.25 * 50))); //cardbg.animate({ zoom: 1 - (mainCard0.data.top / (6.25 * 50)) }); //cardbg.finish().animate({ zoom: 1 - (mainCard0.data.top / (6.25 * 50)) }, 40); //cardbg.css(transform, scale( + (1 - (mainCard0.data.top / (6.25 * 50))) + )); }; }; var scrollSum 0; // negative is down, positive is up var lastScrollDir down; var vendor window.navigator.vendor; var scrollSumClear 0; var lockoutTimer 0; var isLocked false; //attach objects to elements for tracking purposes function MouseWheelHandler(e, direction) { //scroll was up, cards move down //scroll was down, cards move up if (autoscroll) { for (var i 0; i scrolling.length; i++) { clearTimeout(scrollingi); } scrolling ; autoscroll false; } var e window.event || e; if (!direction) { direction (e.wheelDelta || -e.detail) > 0 ? up : down; } else { scrollSum 0; // ignore scroll prevention } if (lastScrollDir ! direction) scrollSum 0; // ignore scroll prevention if (vendor ! Google Inc.) scrollSum 0; // ignore scroll prevention lastScrollDir direction; // attempt to reduce smooth scroll effect // when the movement sum is 0, scroll, however // when there has been movement, dont scroll until 100 wheel units var movement (e.wheelDelta || -e.detail); if (scrollSum ! 0 && false) { if (isLocked) return; clearTimeout(lockoutTimer); lockoutTimer setTimeout(function () { isLocked false; }, 50); isLocked true; clearTimeout(scrollSumClear); scrollSumClear setTimeout(function () { scrollSum 0; }, 750); scrollSum + parseInt(movement); if ((lastScrollDir up && scrollSum 120) || (lastScrollDir down && scrollSum > -120)) { return false; } else { scrollSum 1; } } else { //scrollSum + parseInt(movement); } if (direction up) { cardManager.scrollUp(); } else { cardManager.scrollDown(); } return false; } $(.burgerMenu select).on(change, function () { var iconSelector .icon. + this.value.split( )0; shouldUpdate false; $(iconSelector).click(); cardManager.scrollTo(this.value.split( )1); $(this).blur(); }); $(.header .logo).click(function () { var iconSelector .icon.whoweare; shouldUpdate false; $(iconSelector).click(); cardManager.scrollTo(.overview); $(this).blur(); }); setTimeout(function () { var start ; if (start ) return; var title $(# + start).find(.cardTitle).text(); $(.scrollTitle).text(title); var iconSelector .icon. + start.split( )0; shouldUpdate false; $(iconSelector).click(); cardManager.scrollTo(start.split( )1, function () { setTimeout(function () { $(.scrollToHolder).fadeOut(); shouldUpdate true; }, 100); }); }, 800); }); }); /script> style> body { margin: 0; overflow-y: hidden; scroll-behavior: auto; } .bodybgfiltertop { position: fixed; width:100%; height:30%; z-index:2; right:0;left:0;top:0; background-image: linear-gradient(rgba(0,0,0,0.28),rgba(255,255,255,0.52)); } .bodybgfilterbottom { position: fixed; width:100%; height:30%; z-index:2; right:0;left:0;top:70%; background-image: linear-gradient(rgba(255,255,255,0.52),rgba(0,0,0,0.28)); } .bodybgfilter { position: fixed; width:100%; height:100%; z-index:2; right:0;left:0;top:0;bottom:0; background-image: linear-gradient(135deg,rgba(255,255,255,0.5),rgba(0,0,0,0.38)); } .predictor { z-index:-1000; position:absolute; height:100%; width:100%; -webkit-transform: translateZ(0); -moz-transform: translateZ(0); -ms-transform: translateZ(0); -o-transform: translateZ(0); transform: translateZ(0); } .textpredictor { position:absolute; top:0; left:0; width:100%; height:100%; z-index:-1000; background-color:transparent; -webkit-transform: translateZ(0); -moz-transform: translateZ(0); -ms-transform: translateZ(0); -o-transform: translateZ(0); transform: translateZ(0); } .scrollbarHolder { position: absolute; top:10vh; left:85%; width:10%; height:80vh; } .scrollbar { position:absolute; top:0; height:100%; width:6%; left:44%; background-color:#5e5e5e; } .scrollIcon { position:absolute; top:0; width:46%; left:25.4%; height:10%; transition: all 0.2s linear; text-align:center; } .cardholder { position:absolute; top:0; width:96%; height:100%; overflow:hidden; will-change: transform; /*this is to enforce the radius*/ -webkit-transform: translateZ(0); -moz-transform: translateZ(0); -ms-transform: translateZ(0); -o-transform: translateZ(0); transform: translateZ(0); border-top-left-radius: 18px; border-bottom-right-radius: 55px; box-shadow: 0px 2px 2px #2c2c2c; left:2%; } #whoweare.cardholder { transform:translateX(0%); transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -webkit-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -moz-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -o-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); } #whatwedo.cardholder { transform:translateX(105%); transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -webkit-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -moz-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -o-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); } #publications.cardholder { transform:translateX(105%); transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -webkit-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -moz-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); -o-transition: all 0.6s cubic-bezier(0.6, 0.04, 0.98, 0.335); } .card { top:0; /*left:1%; right:1%;*/ height:100%; /*width:98%;*/ width:100%; position: absolute; overflow: hidden; background-color:lightgray; will-change: transform; -webkit-transform: translateZ(0); -moz-transform: translateZ(0); -ms-transform: translateZ(0); -o-transform: translateZ(0); transform: translateZ(0); z-index:0; } .cardtext { position: absolute; z-index:100; top:0;left:0; padding-left:1%; padding-right:1%; /*padding-top:50px;*/ padding-top:10%; padding-bottom:150px; font-size:1.2em; } .floatrow{ display: flex; width: 97%; align-items: center; margin: 0.5% 0.2% 5% 3%; } .rowheader{ font-size: calc(3vw + 0.8vh); text-align: center; } hr.greencenter{ border:none; margin-top:0.2em; height:3px; width:85%; border-radius:55%; background: radial-gradient(circle, rgba(88,140,105,1) 0%, rgba(255,255,255,1) 65%, rgba(88,140,105,1) 70%, rgba(255,255,255,1) 75%, rgba(88,140,105,1) 80%, rgba(255,255,255,1) 85%, rgba(88,140,105,1) 90%, rgba(255,255,255,1) 95%, rgba(88,140,105,1) 100%); } .animate { transition: all 0.15s cubic-bezier(0.215, 0.61, 0.355, 1); -webkit-transition: all 0.15s cubic-bezier(0.215, 0.61, 0.355, 1); -moz-transition: all 0.15s cubic-bezier(0.215, 0.61, 0.355, 1); -o-transition: all 0.15s cubic-bezier(0.215, 0.61, 0.355, 1); will-change: transform; -webkit-transform: translateZ(0); -moz-transform: translateZ(0); -ms-transform: translateZ(0); -o-transform: translateZ(0); transform: translateZ(0); } .cardbg, .cardfilter, .cardTexture { top:0;left:0;right:0;bottom:0; height:100%;width:100%; position: absolute; } .cardbg { /*zoom: 100%; -webkit-transform: scale(1); -moz-transform: scale(1); -ms-transform: scale(1); -o-transform: scale(1); transform: scale(1);*/ } .cardTexture > div { position: absolute; border-radius:4px; /*border: 1px solid rgba(255,255,255,0.35); box-shadow: 0px 0px 1px 2px rgba(255,255,255,0.2);*/ border: 1px solid rgba(255,255,255,0.2); box-shadow: 0px 0px 1px 2px rgba(0,0,0,0.08); background-image: linear-gradient(transparent, rgba(0,0,0,0.04), rgba(255,255,255,0.04), rgba(0,0,0,0.04), transparent); z-index:10; } .cardTexture .textureOne { top: 10%;height:25%; left:10%;width:20%; } .cardTexture .textureTwo { top: 17%; height: 10%; left: 23%; width: 60%; } .cardTexture .textureThree { top: 22%; height: 60%; left: 20%; width: 50%; } .cardTexture .textureFour { top: 74%; height: 15%; left: 27%; width: 45%; } .cardTexture .textureFive { top: 55%; height: 40%; left: 65%; width: 17%; } #whoweare .cardfilter { background-image: linear-gradient(rgba(0,0,0,0.38),rgba(0,0,0,0.45)); } #whatwedo .cardfilter { background-image: linear-gradient(rgba(0,0,0,0.38),rgba(0,0,0,0.45)); } #whoweare .cardText { color:white; text-shadow: 1px 1px 2px black, 0 0 1em black, 0 0 0.2em black; font-weight: bold; } #whatwedo .cardText { color:white; text-shadow: 1px 1px 2px black, 0 0 1em black, 0 0 0.2em black; font-weight: bold; } .cardText a { text-shadow: 1px 1px 2px white, 0 0 1em white, 0 0 0.2em white; } .overview .cardbg{ background-image: url(../Content/ustgcmap.gif); background-size: cover; background-position: center; 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font-size:3em; } .burgerMenu{ cursor: pointer; } .burgerMenu select:focus{ outline: none; } .burgerMenu select{ cursor: pointer; color: transparent; background-color: transparent; } .burgerMenu optgroup, .burgerMenu option{ color: black; } /style> !-- Global site tag (gtag.js) - Google Analytics --> script async srchttps://www.googletagmanager.com/gtag/js?idUA-112171015-1>/script> script> window.dataLayer window.dataLayer || ; function gtag() { dataLayer.push(arguments); } gtag(js, new Date()); gtag(config, UA-112171015-1); /script> script>$(script).remove();/script> /head> body> div classchevlefttarget styledisplay:none;>/div> div classchevron chev-left-1 rotate180 styledisplay:none;>/div> div classchevron chev-left-2 rotate180 styledisplay:none;>/div> div classchevrighttarget>/div> div classchevron chev-right-1>/div> div classchevron chev-right-2>/div> div classchevron chev-bottom-1 rotate90>/div> div classchevron chev-bottom-2 rotate90>/div> div classsplashscreen> div classintroholder> img classintrologo src../Content/main logo trans.gif /> div classintro> span>T/span>span>i/span>span>s/span>span>s/span>span>u/span>span>e/span>span>-/span>span>G/span>span>r/span>span>o/span>span>w/span>span>n/span>span> /span>span>C/span>span>o/span>span>r/span>span>p/span>span>o/span>span>r/span>span>a/span>span>t/span>span>i/span>span>o/span>span>n/span> /div> div classlogocover>/div> /div> div classloadingfooter> div> loading... /div> div classsquares> div>/div> div>/div> div>/div> /div> /div> /div> div classbodybgfilter>/div> div classheader booth> div classburgerMenu style bottom: 35%; position: absolute; top: 15%; margin: auto 0; overflow: hidden; width: 4%; left: 2%; > div style position: absolute; border-radius: 10%; width: 100%; height: 100%; pointer-events: none; background-image: linear-gradient(rgba(49, 114, 71, 1) 20%, transparent 20%, transparent 40%, rgba(150, 150, 150, 1) 40%, rgba(150, 150, 150, 1) 60%, transparent 60%, transparent 80%, rgba(107, 107, 107, 1) 80%, rgba(107, 107, 107, 1) 100%); >/div> select style width: 100%; height: 100%; border: none; > option style>/option> optgroup labelWho We Are> option valuewhoweare .overview>Company Overview/option> option valuewhoweare .aboutus>About Us/option> option valuewhoweare .facility>Facility/option> option valuewhoweare .contactus>Contact Us/option> /optgroup> optgroup labelWhat We Do> option valuewhatwedo .pistachio>Pistachios/option> option valuewhatwedo .coffee>Coffee/option> option valuewhatwedo .walnut>Walnuts/option> option valuewhatwedo .plantlet>Plantlets/option> /optgroup> optgroup labelPublications> option valuepublications .chapter>Integrating Automation Technologies with Commercial Micropropagation/option> option valuepublications .research>Advances in Research Models for Commercial Micropropagation Systems/option> option valuepublications .germplasm>Principals of Germplasm and Disease-Indexed Line Maintenance/option> option valuepublications .viruselite>Maintenance of Virus-Elite Tissue Cultures/option> /optgroup> /select> /div> img classlogo src../Content/main logo trans.gif /> div classwhoweare icon active data-namewhoweare> img classimgico src../Content/whoweare.png /> span>WHO WE ARE/span> /div> div classwhatwedo icon data-namewhatwedo> img classimgico src../Content/whatwedo.png /> span>WHAT WE DO/span> /div> div classpublications icon data-namepublications> img classimgico src../Content/publications.png /> span>PUBLICATIONS/span> /div> /div> div classpageHolder>div classcardholder animate idwhoweare>div classoverview card animate> div classslideNumber>1/4/div> div classcardTitle>a href/>COMPANY OVERVIEW/a>/div>div classcardbg>/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/ target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionCompany Overview&titleTissue-Grown Corp&urlhttp://www.tissuegrown.com target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corp&urlhttp://www.tissuegrown.com target_blank> img src../Content/twitter.png> /a> /div> div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> div stylefloat:left;width:65%;> p> Tissue-Grown Corporation was founded in 1986 and has an established business in the propagation of disease-indexed horticultural crops. /p> p> Tissue-Grown is a new type of technology company, combining commercial micropropagation and greenhouse production expertise with the disciplines of cell genetics and cell physiology. /p> /div> div stylefloat:right;width:35%;> img src../Content/collagebottom.gif stylemax-width:100%;max-height:100%;/> /div> div stylefloat:left;width:35%;clear:both;> img src../Content/collagetop.gif stylemax-width:100%;max-height:100%;/> /div> div stylefloat:right;width:64%;padding-left:1%;> p> The core business focuses on the mass production of fruit and nut tree rootstocks including span classnavlink data-navwhatwedo .pistachio>pistachio/span>, span classnavlink data-navwhatwedo .coffee>coffee/span>, span classnavlink data-navwhatwedo .walnut>walnut/span>, and cherries. /p> p> Tissue-Grown Corporation uses best practice methods and research and development investments to be a forward thinking green company. Due to these guidelines Tissue-Grown Corp. has grown rapidly and is actively seeking expansion, investments, commercial research partners, and new high volume projects. /p> /div> /div>/div>div classaboutus card animate> div classslideNumber>2/4/div> div classcardTitle>a href/Browse/About>ABOUT US/a>/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/About target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionAbout Us&titleTissue-Grown Corps About Us page&urlhttp://www.tissuegrown.com/Browse/About target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corps About Us page&urlhttp://www.tissuegrown.com/Browse/About target_blank> img src../Content/twitter.png> /a> /div> div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> p> span stylefont-size:1.4em;>Carolyn J. Sluis/span> span> is an industry leader in plant tissue culture and has been working in the field of plant micropropagation and potato tissue culture since 1975. She is listed as inventor on five patent applications (issued and pending) regarding inventions of new tissue culture techniques. Ms. Sluis is the principal founder and Chief Executive Officer of Tissue-Grown Corporation./span> br>Ms. Sluis formal training comes from education in plant environmental biology, at University of California Santa Barbara and San Diego campuses. She also completed course work with the father of commercial plant tissue culture Dr. Toshio Murashige, at the University of California, Riverside. From 1975-1977, Ms. Sluis was employed as the tissue culture manager for K.M. Nurseries in California, one of the first commercial tissue culture laboratories. In 1978, Ms. Sluis started a tissue culture laboratory for NPI (then Native Plants, Inc.) a biotechnology company based in Salt Lake City, Utah. Her research there resulted in development of production systems for a number of endangered species and unusual native species. She initiated the potato project at NPI, which produced the first commercial virus tested potato plantlets in the United States. From 1981-1986 Ms. Sluis was a Research Scientist and Research Group Leader at Plant Genetics, Inc. (now a division of Calgene, Inc.). She led the group responsible for the development of one of Plant Genetics, Inc.s key products: potato minitubers. In addition to research, she pioneered commercial potato minituber production, now an industry standard for the production of elite seed potatoes. Ms. Sluis has consulted internationally with companies in Thailand, Japan, France, Australia, Germany and the Netherlands. She is a key consultant for Floratiss, B.V. in the Netherlands, which was one of the worlds largest lily bulb micropropagation laboratories in Portugal and South Africa. /p> p> span stylefont-size:1.4em;>Travis Johansen/span> has worked with Tissue-Grown since 1999. Mr. Johansen is a graduate of Hawaii Pacific University, earning a Bachelors Degree of Science in Computer Science in 2010, and has studied at Santa Barbara City College and the University of California, Santa Barbara. He currently designs, implements, and maintains software and information systems; oversees production models for delivery goals; and handles administrative tasks at Tissue-Grown. /p> p> span stylefont-size:1.4em;>Brett Gytri/span> is a graduate of UCSB with a degree in Pharmacology, and is a researcher at Tissue-Grown Corporation. Mr. Gytri has worked on and had success with research at Tissue-Grown including increasing the rooting rate for pistachio rootstock, increasing the efficiency of plant production in the lab, developing new media through Design of Experiment, and testing proprietary methods. /p> /div>/div>div classfacility card animate> div classslideNumber>3/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/Facility target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionFacility&titleTissue-Grown Corps Facility page&urlhttp://www.tissuegrown.com/Browse/Facility target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corps Facility page&urlhttp://www.tissuegrown.com/Browse/Facility target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/Facility>FACILITY/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> p>Tissue-Grown Corporation operates a 9,300 square foot laboratory designed around 3 primary tissue culture production subdivisions, in Santa Paula, California. The laboratory encompasses 3000 sq.ft. of media preparation, dishwashing, autoclaving and storage areas./p> p>The conventional micropropagation and the photoautotrophic miniplug production divisions each have 2400 sq.ft. of transfer hoods and large climate-controlled rooms with rolling culture shelves. Each culture room can hold over 1 million plantlets at any point in time, and for the past several years the lab has operated with at least 1 million tree cultures in inventory at any given time./p> p>There are several research areas for designated projects in photoautotrophy, with controlled CO2 rooms, innovative lighting research for photomorphogenesis effects, and chopper-lighting (a hrefwww.chopperlight.de>www.chopperlight.de/a>) for light color control and energy savings./p> p>On site, Tissue-Grown also has refrigerated coolers, research and propagation greenhouses, and warehouse space. The greenhouses contain close to 20,000 square feet of bench space, use extensive proprietary technology, and are managed by state of the art environmental controls./p> /div>/div>div classcontactus card animate> div classslideNumber>4/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/Contact target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionContact&titleTissue-Grown Corps Contact page&urlhttp://www.tissuegrown.com/Browse/Contact target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corps Contact page&urlhttp://www.tissuegrown.com/Browse/Contact target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/Contact>CONTACT US/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> p>Tissue-Grown Corporation is located in beautiful a hrefhttp://en.wikipedia.org/wiki/Santa_Paula,_California>Santa Paula, California/a>./p> p>Santa Paula is in Southern California and is known as the Citrus Capitol of the World because of its lush top soil and Mediterranean climate./p> p>Shipping, both nationally and internationally, is possible via Los Angeles International Airport, just one hour away. Tissue-Grown is very experienced in the management of international shipments - such as working with import permits, phytosanitary certificates, commercial invoices, and customs brokers./p> div styleposition:relative;left:0;right:0;margin:0 auto;width:80%;> div> div stylefont-weight:bold;>Tissue-Grown Corporation/div> div stylemargin:8px;>15245 W Telegraph Rdbr> Santa Paula,CA 93060br> (805) 525-1975/div> /div> a target_blank hrefhttps://maps.google.com/maps?ll34.335454,-119.096658&z9&tm&hlen-US&glUS&mapclientembed&cid11883610472492684275> img src../Content/map.gif stylemax-width:100%/> /a>br> a target_blank hrefhttps://maps.google.com/maps?ll34.335454,-119.096658&z9&tm&hlen-US&glUS&mapclientembed&daddrTissue-Grown%20Corporation%2015245%20W%20Telegraph%20Rd%20Santa%20Paula%2C%20CA%2093060@34.335454,-119.096658>Directions/a> ? ? a target_blank hrefhttps://maps.google.com/maps?ll34.335454,-119.096658&z9&tm&hlen-US&glUS&mapclientembed&cid11883610472492684275>View larger map/a> /div>br> div> div>Send an email to/div> br> div stylemargin:8px;>Carolyn Sluis (President): carolynsluis at tissuegrown.com/div> div stylemargin:8px;>Travis Johansen (Vice President): admin at tissuegrown.com/div> br> div stylemargin:8px;>Brett Gytri (Research Director): brett.gytri at tissuegrown.com/div> br> div stylemargin:8px;>Sales / Ordering: sales at tissuegrown.com/div> div stylemargin:8px;>New Crop Development: newcrops at tissuegrown.com/div> div stylemargin:8px;>General Questions: inquiries at tissuegrown.com/div> /div> /div>/div> /div>div classcardholder animate idwhatwedo>div classpistachio card animate> div classslideNumber>1/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/Pistachio target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionPistachios&titleTissue-Grown Corps Pistachio page&urlhttp://www.tissuegrown.com/Browse/Pistachio target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corps Pistachio page&urlhttp://www.tissuegrown.com/Browse/Pistachio target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/Pistachio>PISTACHIO/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> div styleposition:relative;> div stylez-index:1;position:relative;width:65%;float:right;background-image:linear-gradient(rgba(255,255,255,0.3),rgba(255,255,255,0.55))> img src../Content/pistac plug.gif stylemax-width:100%; /> /div> div stylefloat:left;width:35%;> div> Tissue-Grown Corporation offers state-of-the-art clonal i>in vitro/i> propagation services for pistachio rootstock trees.br/>br/>We have a proven track record of mass producing pistachios, having shipped 50,000 trees per week for extended durations.br/>br/>To date we have produced over 15 million pistachio rootstock trees. /div> /div> /div> /div>/div>div classcoffee card animate> div classslideNumber>2/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/Coffee target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionWalnuts&titleTissue-Grown Corps Coffee page&urlhttp://www.tissuegrown.com/Browse/Coffee target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corps Coffee page&urlhttp://www.tissuegrown.com/Browse/Coffee target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/Coffee>COFFEE/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> div styleposition:relative;> div classrowheader> High Yielding Coffee Trees hr classgreencenter /> /div> div classfloatrow> div stylefloat:left;width:55%;clear:both;padding-right:2%> p> The coffee trees produced in liners at Tissue-Grown are 100% started from tissue culture.br />br />Not only does this allow us to meet a high volume demand for orchard replacement or planting, but it also produces a tree which is vigoursly growing and free from disease or virus. /p> div> We currently have several cutting edge varieties available, and can acquire others if desired. ul> li>Milenio - High yielding and rust resistant br />sup>sub>(a hrefhttps://varieties.worldcoffeeresearch.org/varieties/milenio target_blank>World Coffee Research Datasheet on Milenio/a>)/sub>/sup>/li> li>Centroamericano - High yielding and rust resistant br />sup>sub>(a hrefhttps://varieties.worldcoffeeresearch.org/varieties/centroamericano target_blank>World Coffee Research Datasheet on Milenio/a>)/sub>/sup>/li> /ul> /div> /div> div stylefloat:right;width:45%;> img src../Content/coffee_liners.jpg stylemax-width:100%;max-height:100%;/> /div> /div> p styletext-align:center;width:80%;margin:10% auto;> span classnavlink data-navwhoweare .contactus>Contact Us/span>, or send an email to sales at tissuegrown.com, to discuss how these new varieties or services can benefit your farm or nursery. /p> div classrowheader> Mass Production Paired With Excellent Cupping hr classgreencenter /> /div> div classfloatrow> div stylefloat:left;width:35%;clear:both;> img src../Content/coffee_rooted.jpg stylemax-width:100%;max-height:100%;/> /div> div stylefloat:right;width:64%;padding-left:1%;> p> Our coffee protocol was developed to help meet the demands that farmers face when planting a crop which must be both resistant to Coffee Leaf Rust and at the same time provide the highest quality cupping available. /p> /div> /div> div classrowheader> State-of-the-art Tissue Culture hr classgreencenter /> /div> div classfloatrow> div stylefloat:left;width:65%;> p> Tissue-Grown has researched and developed a robust i>in-vitro/i> model for the production of coffee in tissue culture. The genesis of this work was in response to the current threat of coffee leaf rust (caused by a hrefhttps://en.wikipedia.org/wiki/Hemileia_vastatrix>Hemileia vastatrix/a>), which our varieties are resistant to. /p> /div> div stylefloat:right;width:35%;> img src../Content/coffee_tc.jpg stylemax-width:100%;max-height:100%;/> /div> /div> /div> /div>/div>div classwalnut card animate> div classslideNumber>3/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/Walnut target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionWalnuts&titleTissue-Grown Corps Walnut page&urlhttp://www.tissuegrown.com/Browse/Walnut target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corps Walnut page&urlhttp://www.tissuegrown.com/Browse/Walnut target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/Walnut>WALNUT/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> div styleposition:relative;> div stylez-index:1;position:relative;width:65%;float:right;background-image:linear-gradient(rgba(255,255,255,0.3),rgba(255,255,255,0.55))> img src../Content/walnutroot trans.gif stylemax-width:100%; /> /div> div stylefloat:left;width:35%;> div> p> Tissue-Grown Corporation offers state-of-the-art clonal i>in vitro/i> propagation services for walnut rootstock. /p> p> Tissue-Grown Corporation has proven the ability to mass produce Walnuts as well, with the capacity to produce and ship both locally and internationally. /p> p> In addition to having completed the shipment of hundreds of thousands of walnuts, Tissue-Grown Corporation is also actively researching the ability to graft this crop prior to the field. /p> /div> /div> /div> /div>/div>div classplantlet card animate> div classslideNumber>4/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/Plantlets target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionPlantlets&titleTissue-Grown Corps Plantlets page&urlhttp://www.tissuegrown.com/Browse/Plantlets target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textTissue-Grown Corps Plantlets page&urlhttp://www.tissuegrown.com/Browse/Plantlets target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/Plantlets>PLANTLETS/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> p> Many plant varieties are difficult to propagate, but have been researched and proven to be superior selections. Tissue-Grown Corporation offers state-of-the-art clonal i>in vitro/i> mass propagation services for hard to propagate crops and varieties. /p> p> With a strong history of developing well researched production systems for difficult crops, we can help you with your difficult variety. /p> p> span classnavlink data-navwhoweare .contactus>Contact us/span> to discuss the possibility of getting started on mass producing your difficult crop. /p> /div>/div> /div>div classcardholder animate idpublications>div classchapter card animate> div classslideNumber>1/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/Chapter target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionVisit&titleIntegrating Automation Technologies With Commercial Micropropagation&urlhttp://www.tissuegrown.com/Browse/Chapter target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textIntegrating Automation Technologies With Commercial Micropropagation&urlhttp://www.tissuegrown.com/Browse/Chapter target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/Chapter>Automation Technologies/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> table> tbody>tr> td> img src../Content/IMG/book/book.gif height222 width150 border0>h5> Pages 231-251/h5> /td> td> b>Integrating Automation Technologies With Commercial Micropropagation/b>br> i>An economic perspective/i>br> br> Written By: b>Carolyn J. Sluis/b>br> br> p stylefont-size: 9px;> Sluis, C.J. 2005. Integrating Automation Technologies With Commercial Micropropagation. IN: u>Plant Tissue Culture Engineering/u>br> Gupta, S.D. and Ibaraki, Y. (eds). pp231-251./p> /td> /tr> /tbody> /table> p stylepadding-left:10%;> a href../Content/Chapter.pdf target_blank>View PDF/a> /p> p> b>Abstract/b>br/> Significant advances have been made in micropropagation technologies at both the laboratory and greenhouse levels which offer promise for future mechanization. Several mechanized or automated processes are already seen in commercial practice. This chapter will discuss the various types of mechanization with an emphasis on commercial applications throughout the world. Sections will be devoted to the individual technologies followed by specific analysis of the advantages and disadvantages of the systems relative to commercial implementation. Batch fermentation coupled with bud cluster growth habit modification, and robotics, guided by vision systems, in both laboratory and greenhouse applications will be reviewed. Significant factors key to the commercialization of automation will be discussed, including: 1) bacterial contamination management, starting with culture indexing; 2) economics, especially as regards seasonal production and competitive labor; and, 3) growth habit modification strategies, focussing on bud clusters versus linear growth habits. Potato tissue culture illustrates the complexity of the economic and biological issues, internationally, and will be discussed in depth as an example of a species which can be micropropagated either via linear growth habit, suitable for robotic manipulations, or as a bud clusters, suitable for indiscriminate chopping. br>br>Keywords: micropropagation, biofermentation, bioreactors, cost accounting, robotics /p> /div>/div>div classresearch card animate> div classslideNumber>2/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/ResearchMethods target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionVisit&titleAdvances in Research Models for Commercial Micropropagation Systems&urlhttp://www.tissuegrown.com/Browse/ResearchMethods target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textAdvances in Research Models for Commercial Micropropagation Systems&urlhttp://www.tissuegrown.com/Browse/ResearchMethods target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/ResearchMethods>Research Models/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> div titleView pdf stylewidth:75%;position:relative;> a hrefhttp://www.tissuegrown.com/Content/Keys%20to%20Developing%20an%20Efficient%20Production%20Scheme.pdf target_blank> img stylemax-width:100%; src../Content/researchslide.gif>br /> span>Keys to Developing an Efficient Production Scheme.pdf/span> /a> /div> p> Originally presented at the a hrefhttp://sivb.org/meetings/2015-in-vitro-biology-meeting-abstracts.html target_blank>2015 In Vitro Biology Meeting of the Society for In Vitro Biology/a> /p> p> Presented by Brett Gytri. Co-authored by Carolyn Sluis. /p> h5>Abstract (a hrefhttp://sivb.org/meetings/wp-content/uploads/2015/05/2015plantsymposia.pdf target_blank>Original Submission: P-1/a>)/h5> p> Researchers in universities across the globe have made great strides toward understanding many of the fundamental underlying principals of plant growth regulation, using the model plant Arabidopsis. /p> p> This has resulted in a wealth of avenues to explore for better control of plant development and morphology during propagation in vitro. The challenge in the current economic marketplace is to create reliable, consistent, high volume plant production systems using the best research tools and the best scientific breakthroughs, while keeping in mind both the economics and the marketability of the end product. /p> p> Experimental design and analysis programs have become increasingly accessible to the tissue culture researcher and can help create multifactorial experimental designs which allow evaluation of many interacting factors in a relatively short time frame simultaneously. /p> p> The steps comprising a tissue culture propagation system can be modeled to produce the maximum number of plants while factoring in the best use of labor and materials. The physical parameters of the culture system: the vessels, gas environment and substrate, play a large role in plant quality. Liquid based systems can be compatible with mechanization. Photoautotrophic systems can improve plant quality and normalcy. /p> p> Blending the many options into a functional economic propagation system can be challenging. However, most commercial plant tissue culture laboratories subculture their plants in small vessels with gelled, sugar-containing media. Why? We will explore the reasons for this and examine the research tools available for attaining the goal of high volume micropropagation of economically viable crops in novel and sometimes unexpected ways. /p> /div>/div>div classgermplasm card animate> div classslideNumber>3/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/TechProc1 target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionVisit&titlePrincipals Of Germplasm And Disease-Indexed Line Maintenance&urlhttp://www.tissuegrown.com/Browse/TechProc1 target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textPrincipals Of Germplasm And Disease-Indexed Line Maintenance&urlhttp://www.tissuegrown.com/Browse/TechProc1 target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/TechProc1>Line Maintenance/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> p>Principals Of Germplasm And Disease-Indexed Line Maintenance/p> p> Once a line has been established as meeting the standards for germplasm it can be maintained for many years if proper care is given to details. The most common mistakes in germplasm maintenance involve line identity, line purity and secondary testing. /p> img srchttps://tissuegrown.com/Content/IMG/t1/1.jpg nametarget idtarget width300 height225> br> u>Line identity/u> p> Each germplasm line should have a double identity system: both an initial source number or code and a name or key identifier. Lines should be maintained in culture vessels which are contained in rigid blocks in the germplasm culture area, such as in plastic trays or racks. Each germplasm block is labeled with all relevant identification: accession number, name or breeding number, description, date of accession, etc. Accession numbers should refer to a log kept with additional relevant information (disease testing status, source or origin of the line, etc.). Each test tube or culture vessel within the block is labeled with the accession number.br>br>Operators who are working with the germplasm do not work with more than one line in the workstation at any one time. Only a portion of the culture base is subcultured at any one time, so that a back up of older cultures is always maintained alongside the newly subcultured test tubes. /p> u>Long term line maintenance/u> p> No germplasm lines are ever derived from left-over propagation runs. Germplasm is a one-way culture base; cultures which exit germplasm can never come back in. They can go out to testing or out to production, but cultures in germplasm must not be contaminated by cultures from other sources. Germplasm should be maintained under minimal conditions, such as growth regulator free medium, to ensure the least amount of genetic change. Ideally, germplasm is maintained in a culture room or area separated from the production culture base. Also, a separate area for new lines and quarantined (untested) lines should be enforced. /p> u>Annual certification of germplasm lines/u> p> Every year a single test tube is divided into 3-4 new test tubes, 3 for testing of key viruses, bacteria, etc. and one for starting the next years germplasm. After 6-8 weeks, when viability and test results have been confirmed, the remaining back-up test tubes in germplasm are destroyed. Annual grow-out for verification of line identity and genetic purity is also sometimes warranted. Slight changes in genetics have been well documented, especially in certain species. /p> u>Propagation or production increases starting with germplasm lines/u> p> For production a single germplasm test tube should be selected for increase. This test tube is divided and increased to meet production needs. A single test tube of this individual is kept back, either for testing or grow-out to verify the line characteristics. /p> /div>/div>div classviruselite card animate> div classslideNumber>4/4/div> div classsharelink> a hrefhttps://www.facebook.com/sharer/sharer.php?uhttp://www.tissuegrown.com/Browse/TechProc2 target_blank> img src../Content/fb.png> /a> a hrefhttps://www.researchgate.net/go.Share.html?descriptionVisit&titleMaintenance of Virus-Elite Tissue Cultures&urlhttp://www.tissuegrown.com/Browse/TechProc2 target_blank> img src../Content/rgate.png> /a> a hrefhttps://twitter.com/intent/tweet?textMaintenance of Virus-Elite Tissue Cultures&urlhttp://www.tissuegrown.com/Browse/TechProc2 target_blank> img src../Content/twitter.png> /a> /div> div classcardTitle>a href/Browse/TechProc2>Virus-Elite Cultures/a>/div>div classcardbg> /div>div classcardfilter>/div>div classcardtexture> div classtextureOne>/div>div classtextureTwo>/div>div classtextureThree>/div>div classtextureFour>/div>div classtextureFive>/div> /div> div classcardtext animate> p>Maintenance of Virus-Elite Tissue Cultures/p> p> A clean culture line can, in theory, become infected or reinfected while in tissue culture. We have never experienced this directly, but have seen it on rare occasion, in our consultations. Here are some possible scenarios: br>br> u>Infection via insects/u> br>br> Infestations of insects in test tube cultures does occur. The most common insects which are capable of thriving in tissue cultures are thrips and mites. Thrips are well known carriers of viruses. Thrip infestations are most hazardous if the original insect goes unnoticed and a population sterile offspring is developed. Sterile thrips can be easily subcultured along with the plantlets, as they are often very tiny and not noticeable. Still, thrips need a source of viruses in order to spread them. So the question of greenhouse or outside source of the viruses in question should always be addressed at the same time. br>br> u>Infection via mechanical transfer/u> br>br> If virus-infected cultures co-exist in the laboratory with the virus indexed cultures, then it is possible to cross contaminate by operator error, typically from poor sterilization of tools between each line. br>br> u>Reinitiation of lines from greenhouse plants or propagation cultures/u> br>br> If a germplasm line has been lost to some catastrophe, such as medium contamination, etc., then it is possible that the line was reinitiated into tissue culture from an infected source, such as a greenhouse plant, and insufficient testing was done to ascertain the virus status of the parent line and its tissue culture derivatives. br>br> u>Infection via mislabeling or line identity errors/u> br>br> A line might appear to be re-infected when in fact it is a mislabeled line. The line in question should probably be grown out and verified as the actual plant it is supposed to be. br>br> u>Infection via testing errors/u> br>br> Always question initial results. Testing labs are not infallible. Always make sure the tests have been repeated as blinds (coded labels) before accepting a result. Always examine the optical density readings for the positive and negative controls for indications of testing accuracy. ELISA based testing is rated on statistical significance in the difference between the positive and negative controls and the sample result. It is sometimes not a black and white answer. Some viruses are more problematic in testing than others. br>br> u>Infection because the line was not perfectly virus-free originally/u> br>br> There are many different viruses and additional viruses may become relevant for testing which were not originally part of the elite line requirements. It is always important to check the prior test results for the viruses in question. Also, it is possible to have high titer negatives initially which rise over time to become positives. This is usually only the case over the first 6 months of a line in tissue culture. br>br> Ideally, lines are tested using the broadest based testing system, such as electron microscopy or POTY virus tests, which can reduce the potential for non-obvious viruses present in the culture base. /p> /div>/div> /div> /div> div classpredictor>/div> div classtextpredictor>/div> div idc styleposition:fixed;top:0;left:0;z-index:10000;background-color:white;>/div> /body> /html>
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